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As a result of the pretreatment protocol using the cell block, the conditions of deparaffinization, decrosslinking, and protein extraction were optimized.

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Spatial analysis was employed to map the degree of T-cell infiltration and exhaustion correlating to T-reg and MDSC expression in the tumor microenvironment.

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Kaplan-Meier survival curves stratified by the baseline differentially expressed proteins were highly predictive of overall survival OS and progression-free survival PFS.

Description: Using a general T-cell expansion protocol, we show that adding a cellular label does not alter the viability or release characteristics of T cells.

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